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The high quality isn’t nice but we determined to publish it anyway. MC703 cells had been transduced twice with retrovirus-containing supernatants within the presence of polybrene (10 μg/mL) . Transduced cells have been used to evaluate the T cell response as described below. RNA was purified from tumor biopsy and preserved at -80 °C.

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  • Prediction of binding to HLA class I molecules of mutated and wild kind peptides.
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  • In our case, along with a binding threshold for all mutated peptides, we distinguished two teams regarding the binding capacity of the WT peptide.
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She is featured within the magazine as one of many swimsuit model and is among the cover models as nicely. Click for the Megan Fox 2023 Sports Illustrated swimsuit photos. I assume this could assist lots of people make well knowledgeable tech decisions. We significantly acknowledge the sufferers for their participation and the Biobank of the University of Navarra for its collaboration. Authors thank Virginia Villar for her help with pattern management. We additionally thank Thomas Blankenstein’s lab for offering MC703 cells.

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RNA sequencing was subcontracted to Macrogen Inc . Briefly, complementary DNA libraries have been constructed from High quality RNA (1 μg) utilizing TruSeq stranded Total RNA Sample Prep Kit. Library was subjected to paired-end sequencing using the NovaSeq platform (10 Gb 100PE 100Mreads/sample).


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